Content of the notes –

• Why protein sequencing?

• Determination of amino acid composition

• Ninhydrin reagent test

• N-terminal amino acid analysis

• Mechanism of N-terminal amino acid analysis

• Limitations

• C-terminal amino acid analysis

• Carboxypeptidase

• Proteolytic enzyme

• Disulphide formation

• Solid phase peptide synthesis

• Steps in solid phase peptide synthesis

• What is protein database?

• Difference between sequence and structure database

Protein sequencing and databases | Biochemistry handwritten notes