Content of the notes –
• Why protein sequencing?
• Determination of amino acid composition
• Ninhydrin reagent test
• N-terminal amino acid analysis
• Mechanism of N-terminal amino acid analysis
• Limitations
• C-terminal amino acid analysis
• Carboxypeptidase
• Proteolytic enzyme
• Disulphide formation
• Solid phase peptide synthesis
• Steps in solid phase peptide synthesis
• What is protein database?
• Difference between sequence and structure database
Protein sequencing and databases | Biochemistry handwritten notes
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