Unit V Spectroscopic Techniques
[A]
UV-Visible Spectroscopy: Introduction, interaction of electromagnetic radiation with matter, statement of Beer’s law and Lambert’s law, absorption of UV radiation by organic molecule leading to different electronic transitions, terms involved in UV-visible Spectroscopy- chromophore, auxochrome, bathochromic shift, hypsochromic shift, hyperchromic shift and hypochromic shift, Instrumentation and basic principle of single beam spectrophotometer, applications of UV-visible spectroscopy.
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