Immunology file with all experiments’ theory, principle, procedure and results for MBBS & biotechnology students

Experiments:

1. Handling of animals like rabbit, mice.
2. Preparation of antigens, immunization, and methods of blood collection, serum preparation and storage.
3. To perform radial immunodiffusion assay.
4. To perform double immunodiffusion assay
5. To perform leishman staining
6. To perform giemsa staining
7. To perform sds page
8. Complement fixation test
9. Isolation and purification of IgG from serum
10. Antibody titre by elisa method
11. Cryopreservation of cells
12. Culture of Hela/J774 cells and phagocytosis
13. Separation of mononuclear cells by ficoll hypaque
14. To perform western blotting