Experiments:
- Handling of animals like rabbit, mice.
- Preparation of antigens, immunization, and methods of blood collection, serum preparation and storage.
- To perform radial immunodiffusion assay.
- To perform double immunodiffusion assay
- To perform leishman staining
- To perform giemsa staining
- To perform sds page
- Complement fixation test
- Isolation and purification of IgG from serum
- Antibody titre by elisa method
- Cryopreservation of cells
- Culture of Hela/J774 cells and phagocytosis
- Separation of mononuclear cells by ficoll hypaque
- To perform western blotting
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