Notes for biochemistry, enzymology and biotechnology

CONTENTS:

3D structure of proteins

• 3d structure of proteins
• conformation
• Peptide bond
• dihedrel angles
• Ramachandran plot
• Alpha helix and beta sheet

Purification of proteins/ isolation of enzymes

•  methods of homogenization
• assay of proteins
• general strategy of protein purification depending on thier characteristics
• Salting in and salting out
• ion exchange chromatography
• electrophoresis
• isoelectric focusing
• 2D gel electrophoresis
• dialysis
• affinity chromatography

Catalytic mechanisms of enzymes

Properties of enzymes

Medical applications of enzyme

Biochemical markers used for different diseses

Isozymes their characteristics and uses